| Qualifier |
PCR_primers |
| Definition |
PCR primers that were used to amplify the sequence. A single /PCR_primers qualifier should contain all the primers used for a single PCR reaction. If multiple forward or reverse primers are present in a single PCR reaction, multiple sets of fwd_name/fwd_seq or rev_name/rev_seq values will be present |
| Value Format |
[fwd_name: XXX1, ]fwd_seq: xxxxx1,[fwd_name: XXX2, ]fwd_seq: xxxxx2, [rev_name: YYY1, ]rev_seq: yyyyy1,[rev_name: YYY2, ]rev_seq: yyyyy2 |
| Example |
<INSDQualifier_value>fwd_name: CO1P1, fwd_seq: ttgattttttggtcayccwgaagt,rev_name: CO1R4, rev_seq: ccwvytardcctarraartgttg</INSDQualifier_value>
<INSDQualifier_value>fwd_name: hoge1, fwd_seq: cgkgtgtatcttact, rev_name: hoge2, rev_seq: cg<i>gtgtatcttact</INSDQualifier_value>
<INSDQualifier_value>fwd_name: CO1P1, fwd_seq: ttgattttttggtcayccwgaagt, fwd_name: CO1P2, fwd_seq: gatacacaggtcayccwgaagt, rev_name: CO1R4, rev_seq: ccwvytardcctarraartgttg</INSDQualifier_value>
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| Comment |
fwd_seq and rev_seq are both mandatory; fwd_name and rev_name are both optional. Both sequences should be presented in 5'>3' order. The sequences should be given in the symbols from Annex B.1, paragraph 1, except for the modified bases; those must be enclosed within angle brackets < >. In XML, the angle brackets < and > must be substituted with < and > since they are reserved characters in XML.
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