| Feature | misc_difference |
| Definition | feature sequence is different from that presented in the entry and cannot be described by any other Difference key (unsure, variation, or modified_base) |
| Optional Qualifiers |
/allele="free text" /clone="free text" /compare=[accession-number.sequence-version] /gene="free text" /gene_synonym="free text" /map="free text" /note="free text" /phenotype="free text" /replace="free text" /standard_name="free text" |
| Comment | the misc_difference feature key should be used to describe variability that arises as a result of genetic manipulation (e.g. site directed mutagenesis); use the replace qualifier to annotate a deletion, insertion, or substitution. |